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KMID : 0043319990220020213
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Archives of Pharmacal Research
1999 Volume.22 No. 2 p.213 ~ p.218
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Antioxidant Flavonoids and Chlorogenic Acid from the Leaves of Erobotrya japonica
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Jung Hyun-Ah
Park Jong-Cheol
Chung Hae-Young
Kim Jong
Choi Jae-Sue
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Abstract
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The antioxidant activity of Eriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at , using 2-thiobarbituric acid (TBA). The methanol extract and its factions of Eriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc and n-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCl gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside from n-BuOH fraction and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.
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KEYWORD
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Eriobotrya japonica, Antioxidant activity, Chlorogenic acid, Flavonoids
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